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An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)

An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)

DOI:
10.3791/64445-v

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06:26 min

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September 07, 2022

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, ,
1Key Laboratory of Plant Molecular Physiology, Institute of Botany,Chinese Academy of Sciences, 2College of Life Science,University of Chinese Academy of Sciences, 3Innovative Academy of Seed Design,Chinese Academy of Sciences

Capitoli

  • 00:05Introduction
  • 00:30Seed Collection
  • 01:10Chloral Hydrate-Based Clearing of Seeds Using a Conventional Protocol
  • 02:21Chloral Hydrate-Based Clearing of Seeds Using the Optimized Protocol
  • 04:11Results: Clearing Tomato (Solanum lycopersicum) Seeds at Different Developmental Stages to Observe the Finer Embryonic Structure
  • 05:56Conclusion

Summary

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The tomato seed is an important model for studying genetics and developmental biology during plant reproduction. This protocol is useful for clearing tomato seeds at different developmental stages to observe the finer embryonic structure.

Tags

Seed DevelopmentTomatoSolanum LycopersicumClearing ProtocolEmbryo ObservationChemical PreparationFruit HarvestingSeed CollectionSeed FixationSeed DehydrationClearing SolutionMicroscopyDisinfectant SolutionSeed Mucilage RemovalSeed Washing

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