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Using a Combination of Indirect Calorimetry, Infrared Thermography, and Blood Glucose Levels to Measure Brown Adipose Tissue Thermogenesis in Humans
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Medicina
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JoVE Journal Medicina
Using a Combination of Indirect Calorimetry, Infrared Thermography, and Blood Glucose Levels to Measure Brown Adipose Tissue Thermogenesis in Humans

Using a Combination of Indirect Calorimetry, Infrared Thermography, and Blood Glucose Levels to Measure Brown Adipose Tissue Thermogenesis in Humans

DOI:
10.3791/64451-v

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04:54 min

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June 02, 2023

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, , , ,
1La Trobe Institute for Molecular Science, Department of Rural Clinical Sciences,La Trobe University, 2School of Biomedical Sciences, Department of Physiology,The University of Melbourne, 3Melbourne Medical School, Department of Rural Health,The University of Melbourne

Capitoli

  • 00:05Introduction
  • 00:45Study Day Stratagem
  • 01:20Parameter Measurements
  • 03:08Results: Assessment of Brown Adipose Tissue Thermogenesis
  • 03:56Conclusion

Summary

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Here, we present a protocol to quantify the physiological significance of the impact of brown adipose tissue (BAT) activity on human metabolism. This is achieved by combining carbohydrate loading and indirect calorimetry with measurements of supraclavicular changes in temperature. This novel approach can help develop a pharmacological target for BAT thermogenesis in humans.

Tags

Keywords: Indirect CalorimetryInfrared ThermographyBlood Glucose LevelsThermogenesisCarbohydrate LoadingCaffeineRespiratory Gas AnalyzerNon-contact ThermometerThermal Imaging CameraSupraclavicular TemperatureCore TemperatureReference RegionSubstrate Oxidation Rates

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