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O形环主动脉束带与传统横主动脉收缩在压力超负荷引起的心脏肥大建模中的比较
JoVE Journal
Medicina
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JoVE Journal Medicina
O-Ring Aortic Banding Versus Traditional Transverse Aortic Constriction for Modeling Pressure Overload-Induced Cardiac Hypertrophy
DOI:

09:24 min

October 06, 2022

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Capitoli

  • 00:04Introduction
  • 00:48Preparation of the O-Ring
  • 01:37Premedication of the Mice and Preparation of the Operating Field
  • 02:10Intubation of the Mice
  • 03:33Surgery and Preparation for Ring Positioning
  • 04:41Ligation of the Transverse Aorta with the O-Ring
  • 05:41Suture and Post-Operative Recovery
  • 06:33Confirmation of Successful Constriction and Right Position of the Ring
  • 08:08Results: Modeling Pressure Overload-Induced Cardiac Hypertrophy Using the O-Ring Aortic Banding in a Murine Model
  • 08:57Conclusion

Summary

Traduzione automatica

本协议描述了小鼠主动脉束带诱导压力超负荷心脏肥大的新技术。对于捆扎,使用具有固定内径的橡胶圈。这项新技术有望为未来的实验提供更少的方差和更可重复的数据。

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