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O-ring aortabånd versus tradisjonell tverrgående aortainnsnevring for modellering av trykkoverbelastningsindusert hjertehypertrofi
JoVE Journal
Medicina
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JoVE Journal Medicina
O-Ring Aortic Banding Versus Traditional Transverse Aortic Constriction for Modeling Pressure Overload-Induced Cardiac Hypertrophy
DOI:

09:24 min

October 06, 2022

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Capitoli

  • 00:04Introduction
  • 00:48Preparation of the O-Ring
  • 01:37Premedication of the Mice and Preparation of the Operating Field
  • 02:10Intubation of the Mice
  • 03:33Surgery and Preparation for Ring Positioning
  • 04:41Ligation of the Transverse Aorta with the O-Ring
  • 05:41Suture and Post-Operative Recovery
  • 06:33Confirmation of Successful Constriction and Right Position of the Ring
  • 08:08Results: Modeling Pressure Overload-Induced Cardiac Hypertrophy Using the O-Ring Aortic Banding in a Murine Model
  • 08:57Conclusion

Summary

Traduzione automatica

Denne protokollen beskriver en ny teknikk for aortabånd hos mus for å indusere trykkoverbelastning hjertehypertrofi. For bånd brukes en gummiring med fast indre diameter. Denne nye teknikken lover mindre varians og mer reproduserbare data for fremtidige eksperimenter.

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