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In Vitro Method to Study Sex-Based Differences in Conjunctival Goblet Cells
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JoVE Journal Biologia
In Vitro Method to Study Sex-Based Differences in Conjunctival Goblet Cells

In Vitro Method to Study Sex-Based Differences in Conjunctival Goblet Cells

DOI:
10.3791/64456-v

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06:28 min

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July 28, 2023

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, ,
1Department of Ophthalmology Schepens Eye Research Institute, Massachusetts Eye and Ear,Harvard Medical School

Capitoli

  • 00:04Introduction
  • 01:40Primary Human Conjunctival Goblet Cell Culture and Passaging
  • 02:52Fura‐2/Acetoxymethyl (AM) Assay for [Ca2+]i Measurement
  • 04:52Results: Measuring [Ca2+]i Concentration in Human Conjunctival Goblet Cells
  • 05:30Conclusion

Summary

Traduzione automatica

Traduzione automatica

Phenol red-free/fetal bovine serum-free medium is a better option than advanced RPMI to eliminate exogenous hormones without altering the normal function of conjunctival goblet cells in the study of sex-based differences.

Tags

In VitroConjunctival Goblet CellsSex-based DifferencesPrimary CultureIntracellular Calcium MeasurementFura-2 AMOcular Surface DiseasesDrug DevelopmentCell CultureTrypsinization

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