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Modeling Human Cerebellar Development In Vitro in 2D Structure
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Neuroscienze
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JoVE Journal Neuroscienze
Modeling Human Cerebellar Development In Vitro in 2D Structure

Modeling Human Cerebellar Development In Vitro in 2D Structure

DOI:
10.3791/64462-v

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06:14 min

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September 16, 2022

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, , , , ,
1Department of Psychiatry,University of Iowa, 2Department of Radiology,University of Iowa, 3Carver College of Medicine,University of Iowa, 4Iowa Neuroscience Institute,University of Iowa, 5Pappajohn Biomedical Institute,University of Iowa

Capitoli

  • 00:04Introduction
  • 00:41Experimental Preparation
  • 01:15Cerebellar Differentiation
  • 03:59Results: Overview of the 3D to 2D Cerebellar Differentiation and Expression of Cerebellar Cell Markers
  • 05:48Conclusion

Summary

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The present protocol explains the generation of a 2D monolayer of cerebellar cells from induced pluripotent stem cells for investigating the early stages of cerebellar development.

Tags

Keywords: 2D StructureIn VitroCerebellar DevelopmentHumanNeuropsychiatric DisordersCost-efficientPulled Glass PipetteIPSCEmbryoid BodiesCerebellar Differentiation MediumFGF2

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