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A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro
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Capitoli
- 00:05Introduction
- 00:50Triple Cell Culture BBB Model Setting
- 03:35Induction of the Oxygen-Glucose Deprivation
- 05:19TEER Measurements
- 06:49Assessment of the BBB Paracellular Permeability
- 07:40Detection of Hypoxia in Live Cells
- 08:41Results: A Method to Set up an In Vitro Reliable Triple Endothelial Cell-Pericyte-Astrocyte Culture BBB Model
- 10:20Conclusion
Here, we describe the method for establishing a triple cell culture model of the blood-brain barrier based on primary human brain microvascular endothelial cells, astrocytes, and pericytes. This multicellular model is suitable for studies of neurovascular unit dysfunction during ischemic stroke in vitro or for the screening of drug candidates.
