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Capitoli
- 00:05Introduction
- 01:02Protocol
- 01:25Coating of the HCS plate
- 02:04Seeding of JIMT-1 EGFP cells
- 04:33Pre-treatment of JIMT-1 EGFP cells with compound library
- 06:06Starting the ADCC assay by adding the effector cells
- 09:16Imaging
- 10:45Image analysis
- 12:24Representative results
- 13:15Discussion
Tags
ADCCAntibody-dependent Cellular CytotoxicityNK CellsGranulocytesMacrophagesEffector CellsFcγR ReceptorsTrastuzumabHER2JIMT-1 CellsNK-92 CellsHigh-content Screening96-well PlateCell CultureCell AttachmentCell CountingTrypan BlueCell SuspensionHigh-throughput ScreeningRobotic ArmPin ToolTest Compounds
