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An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue
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JoVE Journal Ricerca sul cancro
An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

DOI:
10.3791/64499-v

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10:33 min

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February 03, 2023

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, , , , , ,
1The First School of Clinical Medicine,Gannan Medical University, 2Laboratory Medicine,First Affiliated Hospital of Gannan Medical University

Capitoli

  • 00:04Introduction
  • 00:46Tissue Dissociation
  • 02:13Differential Ultracentrifugation
  • 04:42Evaluation of Enrichment Quality
  • 09:04Results: Small Extracellular Vesicles Characterization and Analysis
  • 10:02Conclusion

Summary

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Here we describe the enrichment of small extracellular vesicles derived from liver cancer tissue through an optimized differential ultracentrifugation method.

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Keywords: Small Extracellular VesiclesEnrichment MethodDifferential UltracentrifugationSEV IsolationPBS WashingSEV Resuspension

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