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Immunology and Infection
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JoVE Journal Immunology and Infection
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
 

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Article DOI: 10.3791/64522-v • 14:05 min • November 4th, 2022
November 4th, 2022 •
1, 2, 1, 3, 4, 3, 1
1Clinical Immunology Research Department of Biochemical Sciences Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca, 2Department of Molecular Biomedicine, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 3CONACYT- Medicine and Surgery Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca, 4Molecular Immunology Research Department of Medicine and Surgery Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca

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Presented here is a protocol for the induction and analysis of in vitro neutrophil extracellular traps (NETs). Quantification of DNA, cathelicidin (LL37), and enzyme activity yielded data that show the variability in the composition and morphology of NETs induced by microbial and chemical stimuli under similar controlled conditions.

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Keywords: Neutrophil Extracellular Traps Morphology Composition Microbial Stimuli Chemical Stimuli Fluorescence Microscopy Blood Collection Density Gradient Centrifugation Neutrophil Isolation Osmotic Shock
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