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离体尿路上皮细胞中机械活化的CA²⁺ 瞬变分析

JoVE Journal
Medicina

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JoVE Journal Medicina

Ex Vivo Analysis of Mechanically Activated Ca²⁺ Transients in Urothelial Cells

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离体尿路上皮细胞中机械活化的CA²⁺ 瞬变分析

DOI:

10.3791/64532-v

•

05:35 min

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September 28, 2022

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Marcelo D. Carattino², Wily G. Ruiz, Gerard Apodaca²

¹Renal-Electrolyte Division, Department of Medicine,University of Pittsburgh, ²Department of Cell Biology,University of Pittsburgh

Capitoli

00:04Introduction
00:31Fabrication of Micropipettes
00:56In Situ Transduction and Isolation of Bladder Mucosa
01:41Mechanical Stimulation of Individual Urothelial Cells and Ca²⁺ Imaging
03:16Data Analysis
04:03Results: Analysis of Mechano-Activated Ca²⁺ Transients in Urothelial Cells
04:49Conclusion

Summary

Traduzione automatica

该协议描述了一种使用荧光Ca^{2 +} 传感器GCaMP5G评估天然尿路上皮细胞中机械激活离子通道功能的方法。

Tags

Ex Vivo Mechanically-activated Ca2+ Transients Urothelial Cells Micropipettes Bladder Urethra Calcium Ion Transients Electrophysiology

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