È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Department of Pharmacology, College of Medicine,The University of Arizona, Tucson, 2Department of Anesthesiology, College of Medicine,The University of Arizona, Tucson, 3Neuroscience Graduate Interdisciplinary Program, College of Medicine,The University of Arizona, Tucson, 4Comprehensive Pain and Addiction Center,The University of Arizona, Tucson, 5Department of Anesthesiology and Pain Management,University of Texas Southwestern Medical Center
Capitoli
- 00:05Introduction
- 00:57First Step: Measure the Baseline of Mechanical Sensitivity Using the Von Frey Test
- 01:53Second Step: Measure the Baseline of Thermal Sensitivity Using the Hargreaves Test
- 02:41Measuring the Baseline of Thermal Sensitivity Using the Hot Plate Test
- 03:47Preoperative Preparation
- 04:30Surgical Procedure and Postsurgical Behavioral Tests
- 06:38Results: Dose‐Dependent Reversal of pSNL‐Induced Mechanical Hypersensitivity by Intraperitoneal Injection of Morphine or Ibuprofen
- 07:36Conclusion
La ligature partielle du nerf sciatique induit une douleur neuropathique chronique de longue durée, caractérisée par des réponses exagérées aux stimuli thermiques et mécaniques. Ce modèle murin de douleur neuropathique est couramment utilisé pour étudier des thérapies innovantes pour la gestion de la douleur. Cet article décrit en détail l’intervention chirurgicale pour améliorer la standardisation et la reproductibilité.
