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Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs
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JoVE Journal Ricerca sul cancro
Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs

Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs

DOI:
10.3791/64564-v

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10:38 min

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December 16, 2022

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, , , , , , ,
1Felsenstein Medical Research Center, 2Davidoff Center, Rabin Medical Center, 3BioInsight Ltd., 4Sackler Faculty of Medicine,Tel Aviv University

Capitoli

  • 00:04Introduction
  • 01:05Generation and Collection of Spheroids
  • 05:24Drug Efficacy Assay (MTT Assay)
  • 08:05Results: Formation of Spheroids From a Primary Colon Cancer Cell Culture, the Effects of Treatments on Spheroids, and the Follow-Up Over Time
  • 10:04Conclusion

Summary

Traduzione automatica

Traduzione automatica

The present protocol describes generating 3D tumor culture models from primary cancer cells and evaluating their sensitivity to drugs using cell-viability assays and microscopic examinations.

Tags

Keywords: 3D Tumor ModelPatient-derived Tumor Samples3D SpheroidsDrug SensitivityPersonalized Cancer TreatmentPrimary Cancer CellsCell CultureAccutase3D Culture MediumUltra-low Attachment Plate

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