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Isolation and Culture of Bone Marrow-Derived Macrophages from Mice
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

DOI:
10.3791/64566-v

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07:12 min

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June 23, 2023

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, , ,
1Department of General Pathology, Institute of Biological Sciences,Federal University of Minas Gerais, 2Department of Cell Biology and Molecular Genetics,University of Maryland

Capitoli

  • 00:00Introduction
  • 01:08Removal of the Femur and Tibia
  • 01:44Isolation of Bone Marrow Progenitors from Tibia and Femur
  • 03:07Macrophage Culture
  • 03:57Harvesting Macrophages
  • 05:39Results: Characterizing the Bone Marrow-Derived Macrophages
  • 06:42Conclusion

Summary

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The present protocol describes the isolation and culture of bone marrow-derived macrophages from mice.

Tags

Bone MarrowMacrophagesIsolationCultureMurineC57BL/6Sterile TechniqueCentrifugationCell SuspensionDMEM/F12

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