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在携带人类肿瘤的人源化小鼠模型中测试癌症免疫疗法

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Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

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在携带人类肿瘤的人源化小鼠模型中测试癌症免疫疗法

DOI:

10.3791/64606-v

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15:24 min

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December 16, 2022

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Jordi M. Lanis*¹, Matthew S. Lewis*¹, Hannah Strassburger, Kristina Larsen, Stacey M. Bagby, Adrian T. A. Dominguez, Juan A. Marín-Jiménez, Roberta Pelanda, Todd M. Pitts, Julie Lang

¹Department of Immunology and Microbiology, School of Medicine,University of Colorado Denver Anschutz Medical Campus, ²Division of Oncology, School of Medicine,University of Colorado Denver Anschutz Medical Campus, ³Department of Medical Oncology,Catalan Institute of Oncology (ICO-L’Hospitalet)

Capitoli

00:04Introduction
01:06Generation of Human Immune System Mice
04:59Testing Human Chimerism in Blood
08:36Harvesting of Mouse Tissues and Tumors
11:18Results: Evaluation of Chimerism, Tumor Growth Measurements, and Analysis of HIS Cells
14:17Conclusion

Summary

Traduzione automatica

该协议概述了用于免疫肿瘤学研究的人免疫系统（HIS）小鼠的产生。介绍了使用该模型在植入该模型中的人类肿瘤上测试人类免疫疗法的说明和注意事项，重点是表征人类免疫系统对肿瘤的反应。

Tags

Humanized Mouse Model Human Tumor Immunotherapy CD34-positive Cells Cord Blood Cell Expansion Cell Separation Flow Cytometry Preclinical Model Immuno-oncology

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