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丁香假单胞菌基因在植物组织中表达的单细胞分析

JoVE Journal
Biologia

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JoVE Journal Biologia

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

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丁香假单胞菌基因在植物组织中表达的单细胞分析

DOI:

10.3791/64614-v

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07:35 min

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October 06, 2022

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José S. Rufián*¹, Nieves López-Pagán*¹, Javier Ruiz-Albert, Carmen R. Beuzón

¹Depto. Biología Celular, Genética y Fisiología, Instituto de Hortofruticultura Subtropical y Mediterránea, Campus de Teatinos,Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC)

Capitoli

00:05Introduction
00:54Plant Preparation
02:14Inoculation of Arabidopsis and Bean Plants
03:53Extraction of Bacteria from the Apoplast
04:55Single-Cell Analysis of the Apoplast-Extracted Bacteria
06:14Results: Analysis of the Apoplast-Extracted Bacteria Obtained from Arabidopsis or Bean Leaves After Bacterial Inoculation
06:57Conclusion

Summary

Traduzione automatica

本协议描述了一种方法，该方法允许对植物质外体内生长的丁 香假单胞菌 种群进行单细胞基因表达分析。

Tags

Keywords: Single-cell Analysis Pseudomonas Syringae Apoplast Plant Inoculation Bacterial Extraction Flow Cytometry Plant Pathogenic Bacteria Beneficial Endocytic Bacteria Arabidopsis Phaseolus Vulgaris LB Plate Optical Density

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