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Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer
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JoVE Journal Ricerca sul cancro
Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer

DOI:
10.3791/64629-v

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06:49 min

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February 10, 2023

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, , ,
1Stem Cells and Cancer Laboratory,Vall d’Hebron Institute of Oncology (VHIO), 2Preclinical Therapeutics Core,University of California San Francisco, 3CIBERONC

Capitoli

  • 00:05Introduction
  • 00:54Derivation of Patient Cells
  • 03:37Orthotopic Injection in Cecum
  • 05:27Results: Histological Analysis of Orthotopic CRC-PDX Tumors and Therapeutic Efficacy of a Testing Drug
  • 06:26Conclusion

Summary

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This protocol describes the orthotopic implantation of patient-derived cancer cells in the cecum wall of immunodeficient mice. The model recapitulates advanced colorectal cancer metastatic disease and allows for the evaluation of new therapeutic drugs in a clinically relevant scenario of lung and liver metastases.

Tags

Orthotopic ImplantationPatient-derived Cancer CellsColorectal CancerAdvanced Metastatic DiseaseMouse ModelCell DissociationCell SortingCell ResuspensionCecum Injection

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