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Immunologia e infezioni

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一种快速、简单、标准化的均质化制备抗原/辅助乳剂以诱导实验性自身免疫性脑脊髓炎的方法

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis

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一种快速、简单、标准化的均质化制备抗原/辅助乳剂以诱导实验性自身免疫性脑脊髓炎的方法

DOI:

10.3791/64634-v

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05:44 min

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December 09, 2022

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B. Thomas Bäckström²

¹The Rausing Laboratory, Autoimmunity Section, Division of Neurosurgery, Department of Clinical Sciences,Lund University, ²Department of Autoimmunity,BTB Emulsions

Capitoli

00:04Introduction
00:59Material Preparation
01:38Preparation of CFA/Peptide Emulsions
03:31Quality Control of Emulsions
04:01Results: Characterization of Emulsions Produced and EAE Induction in Immunized Mice
05:02Conclusion

Summary

Traduzione automatica

为了诱导实验性自身免疫性脑脊髓炎，一种多发性硬化症的动物模型，用含有自身抗原的油包水乳剂对小鼠进行免疫并完成弗氏佐剂。虽然存在用于制备这些乳液的几种方案，但本文介绍了一种用于乳液制备的快速、简单和标准化的均质化方案。

Tags

Homogenization Method Antigen/adjuvant Emulsion Experimental Autoimmune Encephalomyelitis EAE CFA Slurry IFA Adjuvant Emulsion Preparation Autoimmune Disease Model Cancer Vaccines

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