Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors <em>in vivo</em> by Fluorescence Resonance Energy Transfer

Mi Sa Vo Phan; Phu Tri Tran; Vitaly Citovsky

doi:10.3791/64656

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Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

JoVE Journal
Biologia

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JoVE Journal Biologia

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

DOI:

10.3791/64656-v

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11:33 min

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October 14, 2022

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Mi Sa Vo Phan, Phu Tri Tran, Vitaly Citovsky

¹Department of Biochemistry and Cell Biology,State University of New York

Capitoli

00:05Introduction
01:02Agroinfiltration
03:21Confocal Microscopy
08:11Results: Specific Interaction of LSH10 with OTLD1 in Nicotiana benthamiana Leaves Detected by SE-FRET and AB-FRET
10:41Conclusion

Summary

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Fluorescence resonance energy transfer (FRET) is an imaging technique for detecting protein interactions in living cells. Here, a FRET protocol is presented to study the association of histone-modifying enzymes with transcription factors that recruit them to the target promoters for epigenetic regulation of gene expression in plant tissues.

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

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