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Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
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JoVE Journal Bioingegneria
Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

DOI:
10.3791/64665-v

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05:49 min

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December 02, 2022

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, , , , ,
1Graduate Program in Bioengineering,University of California, Berkeley and University of California, San Francisco, 2Department of Mechanical Engineering,University of California, Berkeley, 3Department of Electrical Engineering and Computer Sciences,University of California, Berkeley

Capitoli

  • 00:04Introduction
  • 00:50Fabricate Device and Measure Cells
  • 03:55Results: wCDI and Cell Recovery Between Various Conditions
  • 05:08Conclusion

Summary

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Presented here is a method to mechanically phenotype single cells using an electronics-based microfluidic platform called mechano-node-pore sensing (mechano-NPS). This platform maintains moderate throughput of 1-10 cells/s while measuring both the elastic and viscous biophysical properties of cells.

Tags

Keywords: Mechano-Node-Pore SensingSingle-cell Viscoelastic MeasurementsMicrofluidic PlatformLabel-freeElectronic MeasurementCell MechanicsCell ViscoelasticityCell PhenotypingCell BehaviorDisease ProgressionDrug Response

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