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功能性破骨细胞与人外周血CD14+ 单核细胞的鉴别
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
DOI:

11:52 min

January 27, 2023

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Capitoli

  • 00:04Introduction
  • 00:56Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Whole Blood
  • 02:34Enrichment of CD14+ Monocytes from PBMCs
  • 04:20Osteoclast Differentiation In Vitro
  • 05:46TRAP Staining for Osteoclasts
  • 07:10Actin Ring Fluorescent Staining
  • 09:40Results: Differentiation of Osteoclasts and Assessing the Function of Mature Osteoclasts
  • 11:12Conclusion

Summary

Traduzione automatica

破骨细胞是体内关键的骨吸收细胞。该协议描述了一种从人外周血单核细胞体 分化的破骨细胞的可靠方法。该方法可作为进一步了解体内平衡和疾病中破骨细胞生物学的重要工具。

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