Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope

Jianjun Zhong; Georgia Gunner; Nils Henninger; Dorothy P. Schafer; Daryl A. Bosco

doi:10.3791/64701

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使用双光子显微镜对闭合颅骨创伤性脑损伤和颅窗小鼠荧光蛋白表达进行活体成像

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JoVE Journal Neuroscienze

Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope

Please note that all translations are automatically generated. Click here for the English version.

使用双光子显微镜对闭合颅骨创伤性脑损伤和颅窗小鼠荧光蛋白表达进行活体成像

DOI:

10.3791/64701-v

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08:25 min

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April 21, 2023

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Jianjun Zhong², Georgia Gunner, Nils Henninger, Dorothy P. Schafer, Daryl A. Bosco

¹Department of Neurology,University of Massachusetts Chan Medical School, ²Department of Neurosurgery,The First Affiliated Hospital of Chongqing Medical University, ³Department of Neurobiology,University of Massachusetts Chan Medical School

Capitoli

00:04Introduction
00:48Traumatic Brain Injury (TBI) Surgery
01:58Cranial Window Implantation Surgery
06:43Results: Intravital Two‐Photon Imaging
07:41Conclusion

Summary

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这项研究表明，向小鼠提供重复性创伤性脑损伤，并同时植入颅窗，以便随后使用双光子显微镜对神经元表达的 EGFP 进行活体成像。

使用双光子显微镜对闭合颅骨创伤性脑损伤和颅窗小鼠荧光蛋白表达进行活体成像

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