Rivista
/
Medicina
/
Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells
JoVE Journal
Medicina
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Medicina
Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells

Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells

DOI:
10.3791/64721-v

•

10:33 min

•

November 18, 2022

•

, , , , ,
1School of Pharmacy,Chengdu Medical College

Capitoli

  • 00:04Introduction
  • 00:56Cell Viability Assay
  • 03:02Screening of Optimal Combination of Two Drugs and its Protective Effect Evaluation
  • 03:37Annexin V-FITC/PI Assay for Apoptosis Rate
  • 05:00Immunofluorescence Detection of Caspase-3 Generation
  • 06:16Immunofluorescence Assay of ROS Level
  • 06:50Western Blot Detection of Protein Expression of Nrf2, p-Akt, Akt, Bcl-2, and Bax
  • 08:11Results: Effects of Drug Combinations on the Normal and Injured PC12 Cells
  • 10:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol provides a combination strategy of two herbs to treat injured PC12 cells. The protocol provides a reference for optimizing the best application mode of traditional Chinese medicine (TCM).

Tags

PC12 CellsHypoxiaHerb CombinationIn VitroCell CultureTrypsinFlow CytometryMTT AssayDMSOHomogenous Design Method

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia

Catheter Ablation in Combination With Left Atrial Appendage Closure for Atrial Fibrillation

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Dual-phase Cone-beam Computed Tomography to See, Reach, and Treat Hepatocellular Carcinoma during Drug-eluting Beads Transarterial Chemo-embolization

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Development of Recombinant Proteins to Treat Chronic Pain

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Vessel-Sparing Microsurgical Longitudinal Intussusception Vasoepididymostomy to Treat Epididymal Obstructive Azoospermia

Using a Combination of Indirect Calorimetry, Infrared Thermography, and Blood Glucose Levels to Measure Brown Adipose Tissue Thermogenesis in Humans

Author Spotlight: Exploring the Complexities of Achilles Tendon Injuries — Research and Future Directions

Read Article