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Utforske de to urt kombinasjonsstrategi for å behandle skadede PC12 celler
JoVE Journal
Medicina
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JoVE Journal Medicina
Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells
DOI:

10:33 min

November 18, 2022

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Capitoli

  • 00:04Introduction
  • 00:56Cell Viability Assay
  • 03:02Screening of Optimal Combination of Two Drugs and its Protective Effect Evaluation
  • 03:37Annexin V-FITC/PI Assay for Apoptosis Rate
  • 05:00Immunofluorescence Detection of Caspase-3 Generation
  • 06:16Immunofluorescence Assay of ROS Level
  • 06:50Western Blot Detection of Protein Expression of Nrf2, p-Akt, Akt, Bcl-2, and Bax
  • 08:11Results: Effects of Drug Combinations on the Normal and Injured PC12 Cells
  • 10:01Conclusion

Summary

Traduzione automatica

Denne protokollen gir en kombinasjonsstrategi av to urter for å behandle skadede PC12-celler. Protokollen gir en referanse for å optimalisere den beste applikasjonsmodusen for tradisjonell kinesisk medisin (TCM).

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