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Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

DOI:
10.3791/64742-v

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06:29 min

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May 05, 2023

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, ,
1Department of Basic Medicine, Wuxi School of Medicine,Jiangnan University

Capitoli

  • 00:04Introduction
  • 00:39Preparation of Recipient Mouse
  • 01:13Isolation and Culture of Interstitial Macrophages (IMs)
  • 03:13Adoptive Transfer of IMs into the Lung Alveoli
  • 05:12Results: Effect of IL‐33‐Stimulated Macrophages on Idiopathic Pulmonary Fibrosis
  • 05:59Conclusion

Summary

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This protocol describes the isolation of pulmonary interstitial macrophages (IMs) and their adoptive transfer after IL-33 stimulation of the lung alveoli in a mouse model, which can facilitate the in vivo study of idiopathic pulmonary fibrosis (IPF).

Tags

Adoptive TransferIL-33MacrophagesBleomycinIdiopathic Pulmonary FibrosisIn VivoClodronate LiposomesAlveolar MacrophagesDMEMCollagenase ACell StrainerCell Lysis BufferHemocytometerInterstitial Macrophages

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