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Thermal Limits Determination for Zooplankton Using a Heat Block
JoVE Journal
Biologia
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JoVE Journal Biologia
Thermal Limits Determination for Zooplankton Using a Heat Block
DOI:

07:16 min

November 18, 2022

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Capitoli

  • 00:04Introduction
  • 00:46Fabrication of the Heat Block
  • 01:45Determination of Thermal Gradient Settings
  • 02:47Thermal Exposure and Live-Dead Enumeration
  • 05:30Computation of LT50
  • 06:10Results: Survivorship and Upper Thermal Limits of Larval Sand Dollars
  • 06:48Conclusion

Summary

Traduzione automatica

The present protocol illustrates the use of commercially available components to generate a stable and linear thermal gradient. Such gradient can then be used to determine the upper thermal limit of planktonic organisms, particularly invertebrate larvae.

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