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A Human Cerebral Organoid Model of Neural Cell Transplantation
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
A Human Cerebral Organoid Model of Neural Cell Transplantation

A Human Cerebral Organoid Model of Neural Cell Transplantation

DOI:
10.3791/64770-v

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08:58 min

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July 21, 2023

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, , ,
1Institut de Recherche en Immunologie et en Cancérologie,Université de Montréal, 2Terry Fox Laboratory,British Columbia Cancer Agency, 3Département de Pathologie et Biologie Cellulaire,Université de Montréal

Capitoli

  • 00:00Introduction
  • 00:36Labeled Cell Injection into the Brain Organoid
  • 02:46Graft Tracking by Live‐Cell Fluorescence Imaging
  • 03:54Histology and Immunofluorescence
  • 06:20Results: Transplantation and Tracking of Labeled Neural Cells into Human Cerebral Organoids
  • 08:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we describe a protocol for the transplantation and tracking of labeled neural cells into human cerebral organoids.

Tags

Keywords: Cerebral OrganoidNeural Cell TransplantationIPSC-derived Neural Progenitor CellsEGFP Labeled CellsCell EngraftmentIn Vitro AssessmentHuman OriginDrug TestingCell TherapyCell SuspensionBasement Membrane MatrixCell InjectionOrganoid CultureMedium Change

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