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1Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology,Shanghai Jiao Tong University, 2Joint Center for Single Cell Biology, School of Agriculture and Biology,Shanghai Jiao Tong University, 3Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education),Shanghai Jiao Tong University
Capitoli
- 00:05Introduction
- 00:36Plant Culture
- 01:20Tissue Preparation and Pretreatment
- 02:37Setting up the Protocol with the Microplate Reader, Establishing the Elicitation System, and Measuring Real-Time ROS Production
- 03:42Results: ROS Induction, the Total Amount of ROS Generated, and ROS Production in the Exposed Cells of the Cut Edge
- 05:07Conclusion
Här beskriver vi en metod för realtidsdetektion av produktion av apoplastiskt reaktivt syre (ROS) i risvävnader i patogenassocierat molekylärt mönsterutlöst immunsvar. Denna metod är enkel, standardiserad och genererar mycket reproducerbara resultat under kontrollerade förhållanden.
