Rivista
/
Genetica
/
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
JoVE Journal
Genetica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

DOI:
10.3791/64798-v

•

08:18 min

•

May 12, 2023

•

, , , , , , , , , , ,
1Department of Vegetable Research,ARO-Volcani Center, 2Department of Vegetable Research,ARO-Newe Ya‘ar Center, 3Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences,ARO- Volcani Center, 4Department of Fruit Science,ARO- Volcani Center

Capitoli

  • 00:04Introduction
  • 00:40Setup for the Initial Separation of Trichomes from the Plant Material
  • 02:08Separation of Trichomes from the Plant Material
  • 03:24Separation of Glandular Capitate Stalked and Sessile Trichomes from Other Trichome Types
  • 05:13Passing Trichomes Through Micro-Sieves of Decreasing Pore Size and Collection of the Desired Trichomes
  • 06:14Results: High-Throughput Isolation & Enrichment of Glandular Capitate Stalked & Sessile Trichomes from Cannabis sativa
  • 07:29Conclusion

Summary

Traduzione automatica

Traduzione automatica

A protocol is presented for the convenient and high-throughput isolation and enrichment of glandular capitate stalked and sessile trichomes from Cannabis sativa. The protocol is based on a dry, non-buffer extraction of trichomes using only liquid nitrogen, dry ice, and nylon sieves and is suitable for RNA extraction and transcriptomic analysis.

Tags

Non-aqueous IsolationEnrichmentGlandular Capitate Stalked TrichomesGlandular Capitate Sessile TrichomesCannabis SativaHigh ThroughputHigh ConcentrationTrichome DensityDry IceLiquid NitrogenMesh SieveExtractionPlant MaterialPlant Processing

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Read Article