/

Biologia dello sviluppo

/

人卵泡中的基因表达分析

JoVE Journal
Biologia dello sviluppo
Author Produced

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biologia dello sviluppo

Gene Expression Analyses in Human Follicles

Please note that all translations are automatically generated. Click here for the English version.

人卵泡中的基因表达分析

DOI:

10.3791/64807-v

•

09:09 min

•

February 17, 2023

•

Melody Devos*¹, Joana Dias Nunes*¹, Isabelle Demeestere²

¹Research Laboratory on Human Reproduction,Université libre de Bruxelles (ULB), ²Fertility Clinic, Erasme Hospital,Université libre de Bruxelles (ULB)

Capitoli

00:05Introduction
00:54Thawing of Cryopreserved Ovarian Tissue
01:58Follicle Isolation
04:26RNA Extraction
07:01Results: Isolation and Gene Expression Analyses in Human Ovarian Follicles
08:16Conclusion

Summary

Traduzione automatica

在这里，我们描述了一个协议，概述了如何从冷冻解冻的皮质组织中分离人类卵巢卵泡以进行基因表达分析。

Tags

Keywords: Gene Expression Ovarian Follicles Germ Cells Gene Expression Analysis Follicle Isolation Enzymatic Isolation Mechanical Isolation Ovarian Cortical Fragment Cryoprotectant Solution Leibovitz 15 Medium Dissection Medium Digestion Medium Scalpel Petri Dish Liquid Nitrogen RT Carbon Dioxide Incubator

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

文化和共同培养小鼠的卵巢和卵巢卵泡

3D器官联合培养模型支持胸腺髓质上皮细胞增殖，分化和混杂基因表达

珠嫁接到发展鸡胚四肢识别信号转导途径影响基因表达

多目标显色全安装<em>原位</em>在比较基因表达域杂交<em>果蝇</em>胚胎

腺病毒基因治疗对糖尿病性角膜病变：对伤口愈合和干细胞标记表达的人体器官培养角膜和角膜缘上皮细胞

细胞谱系分析和基因功能研究使用双点MARCM

在嵌合斑马鱼胚胎的个别视网膜祖细胞转基因表达的体内成像来研究细胞非自治的影响

斑马鱼急性酒精性肝损伤的组织学分析

体外模拟微重力下小鼠腔卵泡生长的研究

细胞聚集法评价果蝇切口与反式配体的结合及其对顺式配体的抑制作用

Read Article