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测试通过微流控混合配制的mRNA-脂质纳米颗粒的 体外体内 效率
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing
DOI:

08:55 min

January 20, 2023

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Capitoli

  • 00:05Introduction
  • 00:50Pre-Formulation Preparation
  • 01:51Preparation of the Lipid and Nucleic Acid Mixes
  • 02:33Microfluidic Formulation of mRNA-LNPs
  • 03:56Post-Formulation Processing and Characterization of the mRNA-LNPs
  • 04:51In Vitro Transfection of HepG2 Cells
  • 05:55In Vivo Evaluation of mRNA-LNPs in Mice Following Tail Vein Injection
  • 07:34Results: Formulation of mRNA-LNPs and the Assessment of In Vitro and In Vivo Performance
  • 08:24Conclusion

Summary

Traduzione automatica

在这里,提出了一种用于配制脂质纳米颗粒(LNP)的方案,该脂质纳米颗粒封装编码萤火虫荧光素酶的mRNA。这些 LNP 在体外 HepG2 细胞和体内 C57BL/6 小鼠中测试 了它们的效力。

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