Rivista
/
Biologia
/
Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis
JoVE Journal
Biologia
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

DOI:
10.3791/64823-v

•

05:34 min

•

June 30, 2023

•

, , , ,
1Centro Universitario de los Lagos,Universidad de Guadalajara, 2Centro Nacional de Recursos Genéticos,Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias

Capitoli

  • 00:04Introduction
  • 00:31Bacterial Sample Preparation and AFM Measurements
  • 03:10Results: Atomic Force Contact Microscopic Analysis of Bacterial Cultures Under Nanoparticle Influence
  • 04:46Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present the application of atomic force microscopy (AFM) as a simple and fast method for bacterial characterization and analyze details such as the bacterial size and shape, bacterial culture biofilms, and the activity of nanoparticles as bactericides.

Tags

Contact Mode Atomic Force MicroscopyMorphological ObservationBacterial Cell Damage AnalysisFixed SampleContact ModeContAI-G ProbesAuto SlopeZ ControllerScan RangeXY PlaneLine By Line LevelingStaphylococcus AureusCocci AggregatesPopulation DistributionCocci Morphology

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

Rapid Analysis and Exploration of Fluorescence Microscopy Images

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy (SEM)

Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate

Characterizing Mechanical Properties of Primary Cell Wall in Living Plant Organs Using Atomic Force Microscopy

Read Article