Rivista
/
Biologia
/
Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

DOI:
10.3791/64825-v

•

04:04 min

•

January 20, 2023

•

, ,
1Department of Physics and Astronomy,University of California, 2Microbiology Program,University of California, 3Biophysics Program,University of California

Capitoli

  • 00:05Introduction
  • 00:44Slide Preparation
  • 01:59Timelapse Fluorescence Microscopy
  • 02:25Results: Visualizing Transposon Activity in Live E. coli Cells
  • 03:30Conclusion

Summary

Traduzione automatica

Traduzione automatica

A protocol is outlined to perform live real-time imaging to quantify how the accessory protein TnpB affects the dynamics of transposition in individual live Escherichia coli cells.

Tags

Real-time QuantificationIS200/IS605 FamilyTnpBTransposon ActivitySingle-cell AnalysisLive Cell ImagingFluorescence MicroscopyExcision DynamicsTransposaseM63 Agarose PadBacterial CultureEpoxy SealTime-lapse Imaging

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/64825?article_type=v

Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay
check_url/it/64825?article_type=v

Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR
check_url/it/64825?article_type=v

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
check_url/it/64825?article_type=v

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
check_url/it/64825?article_type=v

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein
check_url/it/64825?article_type=v

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity
check_url/it/64825?article_type=v

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
check_url/it/64825?article_type=v

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
check_url/it/64825?article_type=v

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
check_url/it/64825?article_type=v

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Read Article