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1Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education,Anhui University of Science and Technology, 2Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes,Anhui University of Science and Technology, 3Anhui Province Engineering Laboratory of Occupational Health and Safety, 4School of Medicine, Department of Medical Frontier Experimental Center,Anhui University of Science and Technology
Capitoli
- 00:04Introduction
- 01:07Preparing the Crystalline Silica Dust (CSD) Suspension
- 01:52Administering the Nasal Drips to the Mouse
- 02:24Performing Hematoxylin and Eosin (HE) Staining
- 03:23Performing Masson Staining and Sirius Red Staining
- 04:27Performing Immunohistochemistry
- 06:10Performing Western Blotting Analysis
- 08:02Results: Investigating the Development of Silicosis in Mice After Repeated CSD Inhalation
- 09:41Conclusion
Dit protocol beschrijft een methode voor het opstellen van een muismodel van silicose door herhaalde blootstelling aan silicasuspensies via een neusinfuus. Dit model kan efficiënt, gemakkelijk en flexibel het pathologische proces van menselijke silicose nabootsen met een hoge herhaalbaarheid en economie.
