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1Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education,Anhui University of Science and Technology, 2Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes,Anhui University of Science and Technology, 3Anhui Province Engineering Laboratory of Occupational Health and Safety, 4School of Medicine, Department of Medical Frontier Experimental Center,Anhui University of Science and Technology
Capitoli
- 00:04Introduction
- 01:07Preparing the Crystalline Silica Dust (CSD) Suspension
- 01:52Administering the Nasal Drips to the Mouse
- 02:24Performing Hematoxylin and Eosin (HE) Staining
- 03:23Performing Masson Staining and Sirius Red Staining
- 04:27Performing Immunohistochemistry
- 06:10Performing Western Blotting Analysis
- 08:02Results: Investigating the Development of Silicosis in Mice After Repeated CSD Inhalation
- 09:41Conclusion
यह प्रोटोकॉल नाक ड्रिप के माध्यम से सिलिका निलंबन के बार-बार संपर्क के माध्यम से सिलिकोसिस के माउस मॉडल को स्थापित करने के लिए एक विधि का वर्णन करता है। यह मॉडल कुशलतापूर्वक, आसानी से और लचीले ढंग से उच्च पुनरावृत्ति और अर्थव्यवस्था के साथ मानव सिलिकोसिस की रोग प्रक्रिया की नकल कर सकता है।
