È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Introduction
- 01:07Pancreatic Dissection
- 02:09Enzymatic and Mechanical Dissociation of the Pancreas
- 05:49Results: FACS Analysis of Isolated Single-Cell Suspension and scRNA-Sequencing
- 07:12Conclusion
Pancreatic metaplastic cells are precursors of malignant cells that give rise to pancreatic tumors. However, isolating intact viable pancreatic cells is challenging. Here, we present an efficient method for pancreatic tissue dissociation. The cells can then be used for single-cell RNA sequencing (scRNA-seq) or for two- or three-dimensional co-culturing.
