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Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons

Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons

DOI:
10.3791/64872-v

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04:26 min

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January 27, 2023

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, , , , ,
1Department of Pharmacology, Graduate School of Medicine,Gunma University, 2Gunma University Initiative for Advanced Research,Gunma University, 3Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences,The University of Tokyo, 4Institute for Drug Discovery Innovation, 5AlzMed, Inc.

Capitoli

  • 00:05Introduction
  • 00:36Plate Coating and Cell Seeding
  • 02:39Neuronal Treatments and Fixation
  • 03:33Results: Neuronal Low-Density Primary Culture Assessment
  • 03:58Conclusion

Summary

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A ready-to-use frozen stock of neurons is a powerful tool for evaluating synaptic functions. Here, we introduce an easy low-density primary culture from frozen stock using a 96-well plate.

Tags

Low-density Primary CultureFrozen StockEmbryonic Hippocampal NeuronsCell CulturePoly-L-lysineMicroelectrode ArrayCell SeedingCell SuspensionCytosine Beta-D-arabinofuranosideGlial Cell Growth

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