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Bestemmelse af vaccineimmunogenicitet under anvendelse af bovin monocytafledte dendritiske celler
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

Bestemmelse af vaccineimmunogenicitet under anvendelse af bovin monocytafledte dendritiske celler

DOI:
10.3791/64874-v

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12:54 min

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May 19, 2023

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, , , , , , ,
1Animal Production and Health Section, Joint Food and Agriculture Organization (FAO)/International Atomic Energy Agency (IAEA) Centre of Nuclear Techniques in Food and Agriculture,International Atomic Energy Agency, 2Teaching and Research Farm Kremesberg, Clinical Unit for Herd Health Management in Ruminants, Department for Farm Animals and Veterinary Public Health,University of Veterinary Medicine, Vienna

Capitoli

  • 00:04Introduction
  • 01:09Production of Naive Bovine Monocyte-Derived Dendritic Cells (MoDCs)
  • 06:01Generation of Antigen-Pulsed MoDCs
  • 07:20MoDC Lymphocyte Co-Culture
  • 08:20Flow Cytometric Analysis
  • 09:47Results: In Vitro Generation of Cattle MoDCs for the Evaluation of Candidate Vaccine Antigens
  • 12:10Conclusion

Summary

Traduzione automatica

Traduzione automatica

Metoden beskriver dannelsen af bovin monocytafledte dendritiske celler (MoDC'er) og deres anvendelse til in vitro-evaluering af antigenkandidater under udviklingen af potentielle veterinærvacciner hos kvæg.

Tags

Keywords: BovineMonocyte-derived Dendritic CellsVaccine ImmunogenicityIn Vitro AssayAntigen-presenting CellsVaccine EfficacyQuality ControlAdjuvant SelectionPeripheral Blood Mononuclear CellsCD14Flow Cytometry

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