Rivista
/
Ricerca sul cancro
/
Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice
JoVE Journal
Ricerca sul cancro
This content is Free Access.
JoVE Journal Ricerca sul cancro
Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice

Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice

DOI:
10.3791/64886-v

•

05:22 min

•

February 24, 2023

•

, , , , , , , ,
1Division of Oncology,Children’s Hospital of Philadelphia, 2Center for Data Driven Discovery in Biomedicine,Children’s Hospital of Philadelphia, 3Division of Neurosurgery,Children’s Hospital of Philadelphia, 4Ben Towne Center for Childhood Cancer Research,Seattle Children’s Research Institute, 5Department of Pediatrics, Seattle Children’s Hospital,University of Washington

Capitoli

  • 00:04Introduction
  • 00:48Mouse Preparation and Surgery Procedure
  • 03:47CAR T Cell Infusion
  • 04:36Results: Efficacy of CAR T Cell Therapy
  • 04:53Conclusion

Summary

Traduzione automatica

Traduzione automatica

Central nervous system (CNS) tumors are the leading cause of cancer-related death in children, and locoregional immune-based therapies are increasingly being tested for patients in clinical trials. This protocol describes methods for locoregional cannula implantation in mice for the preclinical evaluation of immunotherapeutic infusions targeting CNS tumors.

Tags

Keywords: Intracranial CannulaCAR T CellLocoregional DeliveryIntraventricular CatheterStereotactic SurgeryStereotaxic ApparatusCranial LandmarksAcrylic ResinRepeated DosingNeuroscience Research

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/64886?article_type=v

A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells
check_url/it/64886?article_type=v

A Spheroid Killing Assay by CAR T Cells
check_url/it/64886?article_type=v

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
check_url/it/64886?article_type=v

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
check_url/it/64886?article_type=v

Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy
check_url/it/64886?article_type=v

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
check_url/it/64886?article_type=v

Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma
check_url/it/64886?article_type=v

Robot Assisted Distal Pancreatectomy with Celiac Axis Resection (DP-CAR) for Pancreatic Cancer: Surgical Planning and Technique
check_url/it/64886?article_type=v

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography
check_url/it/64886?article_type=v

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Read Article