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Establishment and Culture of Patient-Derived Breast Organoids
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JoVE Journal Ricerca sul cancro
Establishment and Culture of Patient-Derived Breast Organoids

Establishment and Culture of Patient-Derived Breast Organoids

DOI:
10.3791/64889-v

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08:29 min

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February 17, 2023

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, , , ,
1Cold Spring Harbor Laboratory,Cold Spring Harbor, 2Genetics Graduate Program,Stony Brook University

Capitoli

  • 00:04Introduction
  • 01:12Establishing Breast Tumor/Normal Organoids from Resected Tissue
  • 03:59Passaging and Expanding Patient-Derived Breast Organoids in Culture
  • 06:39Results: Morphology and Growth Rate of Patient-Derived Breast Tumor Organoid Lines
  • 07:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

A detailed protocol is provided here for establishing human breast organoids from patient-derived breast tumor resections or normal breast tissue. The protocol provides comprehensive step-by-step instructions for culturing, freezing, and thawing human patient-derived breast organoids.

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Keywords: Patient-derived OrganoidsBreast Tumor Organoids3D In Vitro ModelCellular HeterogeneityCollagenase IVRed Blood Cell LysisBasement Membrane MatrixAdDF+ Medium

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