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通过表皮表型评分 鉴定 参与气孔发育的基因
JoVE Journal
Biologia
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JoVE Journal Biologia
Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring
DOI:

05:22 min

January 20, 2023

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Capitoli

  • 00:04Introduction
  • 00:46Sampling and TBO Staining of Arabidopsis Cotyledons
  • 01:58Peptide Treatment and Imaging
  • 03:24Results: The Effect of the Incubation Time with TBO on the Genotypes, TBO-Stained Epidermis Images, and Confocal Images of Cotyledon Epidermis with or without Peptide Treatment
  • 04:27Conclusion

Summary

Traduzione automatica

本文描述了两种不使用表皮剥皮的表型方法来表征控制气孔发育的基因。第一种方法演示了如何使用甲苯胺蓝O染色植物表皮分析气孔表型。第二种方法描述了如何识别气孔配体并监测其生物活性。

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