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从新生儿啮齿动物中分离和培养前庭和螺旋神经节 Somata 以进行膜片钳记录

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings

Please note that all translations are automatically generated. Click here for the English version.

从新生儿啮齿动物中分离和培养前庭和螺旋神经节 Somata 以进行膜片钳记录

DOI:

10.3791/64908-v

•

11:05 min

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April 21, 2023

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Megana R. Iyer, Christopher Ventura, Daniel Bronson, Nathaniel Nowak, Katherine Regalado, Radha Kalluri

¹Caruso Department of Otolaryngology Head & Neck Surgery, Zilkha Neurogenetics Institute, Hearing and Communications Neuroscience Training Program,University of Southern California

Capitoli

00:00Introduction
01:09Fabricating Trituration Pipettes
01:52Extracting the Superior Vestibular Ganglion
04:01Tissue Dissociation and Plating
05:24Extracting the Spiral Ganglion
06:58Patch-Clamp Recordings
08:31Results: Voltage-Clamp Protocols to Measure Diverse Groups of Ionic Currents and Diverse Firing Patterns
10:25Conclusion

Summary

Traduzione automatica

这里介绍的方法提供了从内耳前庭神经节和螺旋神经节神经元解剖、解离、培养和膜片钳记录的详细说明。

Tags

Keywords: Vestibular Spiral Ganglion Patch-clamp Neonatal Rodents Auditory Afferent Neurons Cell Bodies Ion Channels Neurotransmitter Receptors In Vitro Voltage-dependent Properties Cell Diversity Dissociation Otic Capsule Auditory Nerve Superior Ganglion Inferior Ganglion Peripheral Nerve Branch Utricle

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