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Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
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Neuroscienze
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JoVE Journal Neuroscienze
Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

DOI:
10.3791/64924-v

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10:02 min

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June 02, 2023

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, , , , , , , ,
1Department of Biochemistry and Molecular Biology, Faculty of Pharmacy,Universidad de Sevilla, 2Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío, CSIC,Universidad de Sevilla

Capitoli

  • 00:04Introduction
  • 00:52Isolation of Fungal β-Glucans
  • 05:22In Vitro Study of β-Glucan-Induced Microglia Stimulation
  • 06:31Results: β-Glucan Purification and Their Potential Effects on Microglia Activation
  • 08:50Conclusion

Summary

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The present protocol describes the purification steps and subsequent studies of four different fungal β-glucans as potential immunomodulatory molecules that enhance the anti-tumoral properties of microglia against glioblastoma cells.

Tags

Beta-glucanFungal PolysaccharidesImmunotherapyGlioblastomaMicrogliaMushroom ExtractsPleurotusHericiumGanodermaSoluble Mushroom PolysaccharidesInsoluble Mushroom PolysaccharidesAlpha-amylaseGlucoamylaseAlkalaseExtractionPurification

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