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Den overfladiske ringere epigastriske arterieaksiale klap til undersøgelse af iskæmiske prækonditioneringseffekter i en rottemodel
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1Vascularized Composite Allotransplantation Laboratory,Massachusetts General Hospital, 2Harvard Medical School, 3Department of Plastic, Reconstructive and Aesthetic Surgery, Rennes University Hospital Center (CHU de Rennes),University of Rennes 1, 4Plastic Surgery Research Laboratory,Massachusetts General Hospital, 5Shriners Children’s Boston, 6Center for Engineering in Medicine and Surgery,Massachusetts General Hospital, 7Department of Plastic Surgery and Hand Surgery,University Medical Center Utrecht, 8Department of Plastic, Reconstructive, and Aesthetic Surgery, Groupe Almaviva Santé,Clinique de l’Alma
Capitoli
- 00:05Introduction
- 00:56Harvesting the Epigastric Flap
- 03:43Vessel Preparation and Ischemia Induction
- 06:42Results: Analyzing Post-Operative Flap Viability
- 08:19Conclusion
Denne protokol beskriver høst, suturering og overvågning af fasciokutane klapper hos rotter, der muliggør god visualisering og manipulation af blodgennemstrømningen gennem de overfladiske ringere epigastriske kar ved hjælp af fastspænding og ligering af lårbenene. Dette er kritisk for undersøgelser, der involverer iskæmisk prækonditionering.
