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Evaluatie van autofagieniveaus in twee verschillende pancreascelmodellen met behulp van LC3-immunofluorescentie
JoVE Journal
Biologia
Author Produced
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JoVE Journal Biologia
Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence
DOI:

08:07 min

April 28, 2023

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Capitoli

  • 00:05Introduction
  • 00:49PANC-1 Cell Preparation and Gemcitabine Treatment
  • 01:56Fixation, Permeabilization, and Blocking the PANC-1 Cells
  • 02:32Antibody Incubation
  • 03:57DAPI Staining, Montage, and Visualization of the Cells
  • 04:51LC3 Dots Quantification
  • 06:13Results: Effect of LC3 Immunofluorescence in Pancreatic Cell Lines
  • 07:26Conclusion

Summary

Traduzione automatica

Het doel van dit protocol is om autofagische niveaus in alvleesklierkanker en pancreasacinaire cellen te bepalen door middel van LC3-immunofluorescentie en LC3-puntkwantificering.

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