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小鼠和人泪腺类器官的建立、维持、分化、遗传操作和移植

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

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小鼠和人泪腺类器官的建立、维持、分化、遗传操作和移植

DOI:

10.3791/65040-v

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10:49 min

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February 03, 2023

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Marie Bannier-Hélaouët², Maarten H. Geurts², Jeroen Korving, Harry Begthel, Hans Clevers²

¹Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW),University Medical Center Utrecht, ²Oncode Institute,Hubrecht Institute

Capitoli

00:04Introduction
00:39Organoid Derivation
03:41Functional Swelling Assay
04:45Organoid Electroporation
06:36Orthotopic Transplantation of Human Lacrimal Gland Organoids
08:17Results: Establishment, Genetic Modification, Differentiation, Functional Characterization, and Transplantation of Lacrimal Gland Organoids
10:18Conclusion

Summary

Traduzione automatica

该协议描述了如何建立，维持，基因修饰，分化，功能表征和移植源自原代小鼠和人体组织的泪腺类器官。

Tags

Keywords: Lacrimal Gland Organoids Adult Stem Cell Regenerative Medicine Dry Eye Disease Extracellular Matrix Cell Culture Mouse Human

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