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Establecimiento, mantenimiento, diferenciación, manipulación genética y trasplante de organoides de ratón y glándula lagrimal humana
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
DOI:

10:49 min

February 03, 2023

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Capitoli

  • 00:04Introduction
  • 00:39Organoid Derivation
  • 03:41Functional Swelling Assay
  • 04:45Organoid Electroporation
  • 06:36Orthotopic Transplantation of Human Lacrimal Gland Organoids
  • 08:17Results: Establishment, Genetic Modification, Differentiation, Functional Characterization, and Transplantation of Lacrimal Gland Organoids
  • 10:18Conclusion

Summary

Traduzione automatica

Este protocolo describe cómo establecer, mantener, modificar genéticamente, diferenciar, caracterizar funcionalmente y trasplantar organoides de la glándula lagrimal derivados de tejido primario de ratón y humano.

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