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利用离子阱质谱和多级碎裂分析对藏药化合物结构进行标准化鉴定

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Chimica

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JoVE Journal Chimica

Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis

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利用离子阱质谱和多级碎裂分析对藏药化合物结构进行标准化鉴定

DOI:

10.3791/65054-v

•

09:24 min

•

March 17, 2023

•

Xing Fu, Yuan Pan, Yili Wang, Zhaoqing Pei, Binjie Xu, Jing Zhang, Jinsong Su

¹State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, ²School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine

Capitoli

00:04Introduction
00:51Abelmoschus manihot Seeds (AMS) Sample Preparation
01:32Mass Spectrometry (MS) Setting
02:12Liquid Chromatography Prerun, Method Establishment, and MS Acquisition
04:07Operating Multiple-Stage Mass Spectrometry
06:10Manual MSⁿ Data Analysis
06:47Results: Multiple-Stage Fragmentation and Structural Analysis of Unknown AMS Compounds
08:37Conclusion

Summary

Traduzione automatica

在这里，我们描述了一种通用方案和设计，可用于鉴定藏药中复杂的天然产物制剂（基质）中的痕量和微量成分。

Tags

Keywords: Tibetan Medicine Compound Structure Analysis Ion Trap Mass Spectrometry Multiple-stage Fragmentation Abelmoschus Manihot Metabolic Intermediates Liquid Chromatography-mass Spectrometry (LC-MS)Sample Preparation Mobile Phase Ion Source

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