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Dual-Dye Optical Mapping of Hearts from RyR2R2474S Knock-In Mice of Catecholaminergic Polymorphic Ventricular Tachycardia
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Biologia
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JoVE Journal Biologia
Dual-Dye Optical Mapping of Hearts from RyR2R2474S Knock-In Mice of Catecholaminergic Polymorphic Ventricular Tachycardia

Dual-Dye Optical Mapping of Hearts from RyR2R2474S Knock-In Mice of Catecholaminergic Polymorphic Ventricular Tachycardia

DOI:
10.3791/65082-v

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09:36 min

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December 22, 2023

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, , , , , , , , , ,
1Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research,Southwest Medical University, 2Department of Cardiology,the Affiliated Hospital of Southwest Medical University, 3Department of Cardiology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 4Department of Pharmacology,University of Oxford

Capitoli

  • 00:04Introduction
  • 01:00Mouse Heart Harvest, Cannulation, and Perfusion
  • 03:14Optical Mapping and Arrhythmia Induction
  • 06:38Results: APD80 and CaTD80 Analysis at 10 Hz Before and After ISO Challenge
  • 08:36Conclusion

Summary

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This protocol introduces dual-dye optical mapping of mouse hearts obtained from wild-type and knock-in animals affected by catecholaminergic polymorphic ventricular tachycardia, including electrophysiological measurements of transmembrane voltage and intracellular Ca2+ transients with high temporal and spatial resolution.

Tags

Keywords: Optical MappingRyR2R2474S Knock-in MiceCatecholaminergic Polymorphic Ventricular TachycardiaMembrane PotentialIntracellular CalciumLangendorff PerfusionExcitation-contraction UncouplingRhod-2 AMRH 237ElectrocardiogramCardiac Arrhythmia

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