È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research,Southwest Medical University, 2Department of Cardiology,the Affiliated Hospital of Southwest Medical University, 3Department of Cardiology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 4Department of Pharmacology,University of Oxford
Capitoli
- 00:04Introduction
- 01:00Mouse Heart Harvest, Cannulation, and Perfusion
- 03:14Optical Mapping and Arrhythmia Induction
- 06:38Results: APD80 and CaTD80 Analysis at 10 Hz Before and After ISO Challenge
- 08:36Conclusion
Detta protokoll introducerar optisk kartläggning av mushjärtan med dubbla färgämnen erhållna från vildtyps- och knock-in-djur som drabbats av katekolaminerg polymorf ventrikulär takykardi, inklusive elektrofysiologiska mätningar av transmembranspänning och intracellulära Ca2+ -transienter med hög tidsmässig och rumslig upplösning.
